Work

Plant regeneration in Curcuma species and assessment of genetic stability of regenerated plants

Year: 2010

Type: article

Abstract: An efficient plant regeneration protocol was developed from rhizomes of two Curcuma species C. longa and C. amada. Response was highly dependent on the season, with above 69 % of culture developing adventitious shoots during spring. Greatest regeneration and multiplication was observed in modified Murashige and Skoog (MS) medium supplemented with 13.31 μM benzyladenine and 2.68 μM α-naphthalene acetic acid (NAA) in C. longa or 2.46 μM indolebutyric acid in C. amada. Effect of sugars and agar at different concentrations were also studied and 2 % maltose and 0.7 % agar were found optimum for shoot multiplication and regeneration. Most plantlets developed roots simultaneously but others formed roots when subcultured in 1/2 MS medium supplemented with 2.68 μM NAA. Plants were successfully hardened in greenhouse with 80 % survival. The genetic purity of micropropagated plantlets was analyzed using RAPD and protein profiles.

Source: Biologia Plantarum

DOI: 10.1007/s10535-010-0077-0

Authors: A. Das, V. Kesari, L. Rangan

Institution: Indian Institute of Technology Guwahati

Language: English

Topic: Plant tissue culture and regeneration

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Zero hunger

Open Access status: bronze

Funders: Ministry of Education, India, Gauhati University, Indian Institute of Technology Guwahati