Year: 2011
Type: article
Abstract: Objective: When examining the effects of acute psychological stress on coagulation responses, researchers have typically used the Dill and Costill (1974) mathematical correction to adjust for stress-induced plasma volume shifts.While the correction is appropriate for adjusting concentrations of large blood constituents, it may be inappropriate for time dependent or functional coagulation assays.Two novel plasma reconstitution techniques for correcting hemoconcentration effects on stress-induced coagulation changes were compared to the Dill and Costill correction.Methods: Blood was collected from 40 men during the last minute of a 20-minute baseline period, a 6minute mental arithmetic stressor and a 20-minute recovery period.For the reconstitution techniques, stressor plasma samples were reconstituted with either the person's own plasma from baseline or physiological saline such that plasma volume at stress became equal to plasma volume at baseline.Results: Plasma volume increased significantly from baseline to stress, but returned to baseline levels at recovery.Uncorrected activated partial thromboplastin time (APTT) decreased, whereas Factor VII clotting activity (FVII:C), FVIII:C, prothrombin time (PT%), and fibrinogen concentration increased significantly from baseline to stress.Arithmetic correction produced a significantly greater decrease in APTT during stress compared to uncorrected APTT, whereas FVII:C, PT%, and fibrinogen concentration were not significantly different from baseline after
Author: Anthony W. Austin
Institution: Ohio University
Language: English
Subfield: Hematology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W200647770