Synthesis of histone messenger RNAs by RNA polymerase II in nuclei from S phase HeLa S3cells
Year: 1978
Type: article
Abstract: Nuclei were isolated from synchronized HeLa S3 cells and transcribed utilizing their endogenous RNA polymerases. Our data suggest that S phase nuclei are capable of synthesizing histone mRNA sequences while nuclei from G1 phase cells are not. Transcription of histone mRNA sequences by S phase nuclei can be abolished completely by low levels of alpha-amanitin (1.0 microgram/ml, a concentration which completely inhibits RNA polymerase II). From these results it appears that transcription of the histone mRNA sequences occurs during the S phase but not during the G1 phase of the cell cycle and that RNA polymerase II is responsible for histone gene readout.
Source: Nucleic Acids Research
DOI: 10.1093/nar/5.5.1515
Authors: S. Detke, J.L. Stein, G.S. Stein
Institution: University of Florida
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
Funder: Kela
OpenAlex ID: W2006887940