Work

SR proteins are sufficient for exon bridging across an intron

Year: 1998

Type: article

Abstract: We have developed a defined system to characterize the role of SR proteins and exonic enhancers in directly promoting splice-site interactions across an intron. Using RNA affinity chromatography, we find that SR proteins alone are sufficient to promote the specific association of the enhancer-containing exon 5 with the adjoining exon 6 from avian cardiac troponin-T. Direct visualization of this exon/exon association by electron spectroscopic imaging shows it to be highly specific. Furthermore, using in vivo characterized mutants of exon 5, we also show that this exon/exon association depends on the splicing enhancer within exon 5. These results suggest a model by which SR proteins may function through exonic enhancers to directly promote exon bridging.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.95.5.2163

Authors: Jeremy M. Stark, David P. Bazett-Jones, Manfred Herfort, Mark B. Roth

Institutions: University of Calgary, Fred Hutch Cancer Center

Language: English

Topic: RNA Research and Splicing

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of General Medical Sciences

Awards: G1733974328, G2888679868, G352412733, Training in Cellular and Molecular Biology, G7761418381