Work

Bioluminescent nanosensors for protease detection based upon gold nanoparticle–luciferase conjugates

Year: 2009

Type: article

Abstract: This communication reports the use of click chemistry to site-specifically conjugate bioluminescent Renilla luciferase proteins to gold nanoparticles (Au NPs) for sensing protease activity. The bioluminescent emission from luciferase was efficiently quenched by Au NPs, but significantly recovered after the proteolytic cleavage.

Source: Chemical Communications

DOI: 10.1039/b915612g

Authors: Young-Pil Kim, Weston L. Daniel, Zuyong Xia, Hexin Xie, Chad A. Mirkin, Jianghong Rao

Institutions: Stanford University, Northwestern University, International Institute for Nanotechnology

Language: English

Topic: bioluminescence and chemiluminescence research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Cancer Institute

Awards: G238157917, G3376281236, Nanomaterials for Cancer Diagnostics and Therapeutics, G6211958678, G7413534593