Work

A Facile Method to Synthesize Histones with Posttranslational Modification Mimics

Year: 2012

Type: article

Abstract: Using an evolved pyrrolysyl-tRNA synthetase-tRNA(Pyl) pair, a Se-alkylselenocysteine was genetically incorporated into histone H3 with a high protein expression yield. Quantitative oxidative elimination of Se-alkylselenocysteine followed by Michael addition reactions with various thiol nucleophiles generated biologically active mimics of H3 with posttranslational modifications including lysine methylation, lysine acetylation, and serine phosphorylation.

Source: Biochemistry

DOI: 10.1021/bi300535a

Authors: Zhiyong U. Wang, Yane-Shih Wang, Pei-Jing Pai, William K. Russell, David H. Russell, Wenshe R. Liu

Institution: Texas A&M University

Language: English

Topic: Organoselenium and organotellurium chemistry

Subfield: Toxicology

Field: Pharmacology, Toxicology and Pharmaceutics

Domain: Life Sciences

Open Access status: green

Funder: National Cancer Institute

Award: Phage Display with Two Genetically Incorporated Noncanonical Amino Acids