Work

3D Multi-isotope Imaging Mass Spectrometry Reveals Penetration of 18O-Trehalose in Mouse Sperm Nucleus

Year: 2012

Type: article

Abstract: The prevalence of genetically engineered mice in medical research has led to ever increasing storage costs. Trehalose has a significant beneficial effect in preserving the developmental potential of mouse sperm following partial desiccation and storage at temperatures above freezing. Using multi-isotope imaging mass spectrometry, we are able to image and measure trehalose in individual spermatozoa. We provide the first evidence that trehalose penetrates the nucleus of a mammalian cell, permitting tolerance to desiccation. These results have broad implications for long-term storage of mammalian cells.

Source: PLoS ONE

DOI: 10.1371/journal.pone.0042267

Authors: Claude P. Lechene, Gloria Y. Lee, J. Collin Poczatek, Mehmet Toner, John D. Biggers

Institutions: Brigham and Women's Hospital, Harvard University, Massachusetts General Hospital

Language: English

Topic: Mass Spectrometry Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $2,382

Funders: National Institutes of Health, National Institute on Aging, National Institute of Biomedical Imaging and Bioengineering, National Center for Research Resources