Simplified generation of targetting constructs using ET recombination
Year: 1999
Type: article
Abstract: ET recombination is a way to engineer DNA in Escherichia coli using homologous recombination. Here we develop the potential of ET recombination in two ways relevant to complex engineering exercises such as building gene targeting constructs. First, a targeting construct was made in a single step. Second, ET recombination was used to place two unique restriction sites at precise positions in a large genomic clone. Subsequently a complex targeting construct was created by ligation with a multifunctional cassette.
Source: Nucleic Acids Research
Authors: P.-O. Angrand, N. Daigle, F. van der Hoeven, H. R. Scholer, A. F. Stewart
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
Funder: Volkswagen Foundation
OpenAlex ID: W2007905386