Response of pollen tube growth and seed set to controlled pollination and their relation to self-incompatibility in different cultivars ofPetunia hybrida
Year: 2009
Type: article
Abstract: Self-incompatibility is a widespread mechanism in flowering plants which prevents self-fertilisation and promotes out-crossing. In this investigation, self-compatible and self-incompatible cultivars of Petunia hybrida Vilm. were discriminated based on the study of pollen tube growth in vivo, seed set percent and index of self-incompatibility (ISI) after self- and cross-pollination. After 48 hours of self- and cross- pollination, the pollen tube growth was studied by aniline blue staining and fluorescent microscopy. Fluorescent microscopy studies showed normal germination and growth of pollen tubes on stigmas but some abnormalities of pollen tube growth were observed in self-pollinated styles in some cultivars. These abnormalities include pollen tube growth inhibition in 2/3 upper part of the style, variable pollen tube diameter in middle and lower part of the style and irregular deposition of callose plugs. ISI studies showed a significant decrease in the seed set of self-pollinated pistils compared with cross-pollinated pistils in some cultivars. The self-pollinated cultivars contain abnormalities in pollen tube growth after self-pollination as well as those with decreasing ISI was defined as self-incompatible in our study.
Source: Grana
Authors: Hakimeh Oloumi, Farkhondeh Rezanejhad
Institution: Shahid Bahonar University of Kerman
Language: English
Topic: Plant Reproductive Biology
Subfield: Molecular Biology
Domain: Life Sciences
SDG: Zero hunger
Open Access status: bronze
OpenAlex ID: W2008024496