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Localization of the human insulin gene to the distal end of the short arm of chromosome 11.

Year: 1981

Type: article

Abstract: The human insulin gene was mapped by hybridization in situ of 3H-labeled recombinant plasmid pHIG900 to chromosome preparations in the presence of 10% dextran sulfate. pHIG900 contains a 900-base pair insert of human genomic DNA that includes the coding region for the C peptide and intervening sequence 2. A significant percentage of hybridized cells (34%) exhibited silver grains on the distal end of the short arm (band p15) of chromosome 11. Furthermore, silver grains on this region represented 26% of all chromosomal label. These results demonstrate chromosomal localization of the human insulin gene to 11p15.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.78.7.4458

Authors: M E Harper, A Ullrich, G F Saunders

Institutions: The University of Texas MD Anderson Cancer Center, The University of Texas System

Language: English

Topic: Genomic variations and chromosomal abnormalities

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Institutes of Health

OpenAlex ID: W2008361300

FWCI: 11.26

Cites: 21

Cited by: 281

Related to: 10