Work

Hormonal regulation of pituitary GH3 cell K+ channels by Gk is mediated by its α‐subunit

Year: 1987

Type: article

Abstract: The resolved alpha-GTP gamma S (alpha*) and beta gamma-subunits of human erythrocyte Gk, the stimulatory regulatory component of hormone-responsive K+ channels, were tested for their potential stimulatory activities on the K+ channel of the endocrine GH3 cell. Concentrations as low as 0.5 pM alpha k* consistently activated K+ channels in isolated membrane patches, and saturating effects were obtained with 50 pM alpha k*. In contrast 2000-4000 pM beta gamma was without effect. We conclude that Gk acts on K+ channels through its alpha-subunit in a manner akin to that of Gs acting on adenylyl cyclase and transducin acting on cGMP-specific phosphodiesterase of photo-receptor cells.

Source: FEBS Letters

DOI: 10.1016/0014-5793(87)80765-2

Authors: Juan Codina, Dagoberto Grenet, Atsuko Yatani, Lutz Birnbaumer, Arthur M. Brown

Institution: Baylor College of Medicine

Language: English

Topic: Ion channel regulation and function

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: National Heart, Lung, and Blood Institute, National Institute of Diabetes and Digestive and Kidney Diseases, Eunice Kennedy Shriver National Institute of Child Health and Human Development

Awards: G5614658440, G8000713856, G972493160