Work

Identification of Arginine Residues in Peptides by 2D-IR Echo Spectroscopy

Year: 2011

Type: article

Abstract: The CN stretching vibrations of the guanidyl group in the arginine dipeptide side chain are examined by two-dimensional infrared spectroscopy. In D(2)O, the spectra display two distinct diagonal peaks. These nearly degenerate modes undergo ultrafast energy transfer. The energy-transfer rate was determined directly from the 2D-IR spectra to be 1/2.1 ps(-1). The cross peaks in 2D-IR arising from the energy transfer provide a definitive identification of arginine in larger proteins. An example of arginine in the transmembrane protein M2, found in influenza viruses, is given.

Source: The Journal of Physical Chemistry A

DOI: 10.1021/jp201794n

Authors: Ayanjeet Ghosh, Matthew J. Tucker, Robin M. Hochstrasser

Institution: University of Pennsylvania

Language: English

Topic: Spectroscopy and Quantum Chemical Studies

Subfield: Atomic and Molecular Physics, and Optics

Field: Physics and Astronomy

Domain: Physical Sciences

Open Access status: green

Funders: National Science Foundation, National Institutes of Health, National Institute of General Medical Sciences, National Center for Research Resources