Work

Comparison of Segger and other methods for segmentation and rigid‐body docking of molecular components in Cryo‐EM density maps

Year: 2012

Type: article

Abstract: Segmentation and docking are useful methods for the discovery of molecular components in electron cryo-microscopy (cryo-EM) density maps of macromolecular complexes. In this article, we describe the segmentation and docking methods implemented in Segger. For 11 targets posted in the 2010 cryo-EM challenge, we segmented the regions corresponding to individual molecular components using Segger. We then used the segmented regions to guide rigid-body docking of individual components. Docking results were evaluated by comparing the docked components with published structures, and by calculation of several scores, such as atom inclusion, density occupancy, and geometry clash. The accuracy of the component segmentation using Segger and other methods was assessed by comparing segmented regions with docked components.

Source: Biopolymers

DOI: 10.1002/bip.22074

Authors: Grigore Pintilie, Wah Chiu

Institution: Baylor College of Medicine

Language: English

Topic: Advanced Electron Microscopy Techniques and Applications

Subfield: Structural Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funder: National Institute of General Medical Sciences

Awards: G2788500095, 3 D Electron Microscopy of Macromolecules