Highly Electrically Insulating Tethered Lipid Bilayers for Probing the Function of Ion Channel Proteins
Year: 2003
Type: article
Abstract: A method is presented to form gold-electrode-tethered lipid bilayers with exceptionally high electrical resistances. Electrical impedance spectroscopy is used to monitor the bilayer incorporation of a ligand-gated ion channel protein and the modulation of its channel activity by the selective binding of an antibody. Due to the low defect density of the tethered membrane, the effect of a few channels can be resolved thus opening the way to single-channel experiments on this highly stable and versatile platform. In turn, a minute quantity of analyte, here antibodies, can be measured, which is of great interest for bioanalytics.
Source: Langmuir
DOI: 10.1021/la034197v
Authors: Samuel Terrettaz, Michael Mayer, Horst Vogel
Institution: École Polytechnique Fédérale de Lausanne
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2008848900