Work

The Effect of Charge-Reversal Amphiphile Spacer Composition on DNA and siRNA Delivery

Year: 2010

Type: article

Abstract: A series of charge-reversal amphiphiles with different spacers separating the headgroup from the hydrophobic chains are described for delivery of DNA and siRNA. Among them, the amphiphiles possessing a glycine spacer (e.g., B-GlyGly) showed effective DNA transfection in CHO and NIH 3T3 cells, as well as siRNA gene knockdown in HepG2 and UASMC cells. Ethidium bromide quenching assays revealed that DNA was released the fastest from the lipoplex of B-GlyGly in the presence of esterase. Also, X-ray diffraction results indicated that the DNA was located between the adjacent lipid bilayers in the lipoplex of B-GlyGly. These distinct features appear to be required for high transfection activity.

Source: Bioconjugate Chemistry

DOI: 10.1021/bc9005464

Authors: Xiao-Xiang Zhang, Carla A. H. Prata, Thomas J. McIntosh, Philippe Barthélémy, Mark W. Grinstaff

Institutions: Boston University, Université de Bordeaux, Inserm, Duke Medical Center

Language: English

Topic: RNA Interference and Gene Delivery

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institute of General Medical Sciences, National Institute of Biomedical Imaging and Bioengineering

Awards: G259431816, Reversing Electrostatic Interactions for Improved Gene Delivery, TRP and AQP Channels: Modulation of Function, Raft Location by Membrane Lipids, G8407109578