Inhibitory Activity of Green Tea (Camellia sinensis) Extract on Some Clinically Isolated Cariogenic and Periodontopathic Bacteria
Year: 2013
Type: article
Abstract: OBJECTIVE: To determine the in vitro inhibitory activity of green tea extract on some clinically isolated cariogenic and periodontopathic bacteria. MATERIALS AND METHODS: Twenty strains of each of Streptococcusmutans, Aggregatibacteractinomycetemcomitans, Porphyromonasgingivalis, and Prevotellaintermedia were isolated from carious teeth and periodontal pockets of patients with dental caries and periodontal diseases. Green tea extract was prepared by aqueous extraction method and diluted from 50 to 1.56 mg/ml. Standard techniques of agar disk diffusion and broth microdilution assays were applied for qualitative and quantitative determinations of antibacterial activity of green tea extract on each isolates. RESULTS: All clinical isolates of S. mutans (100%) were sensitive to green tea extract at concentrations 6.25, 12.5, 25, and 50 mg/ml producing inhibition zones ranging from 10 to 38 mm. All periodontopathic isolates (A. actinomycetemcomitans, n = 20, P. intermedia, n = 20, and P. gingivalis, n = 20) (100%) tested were sensitive to 12.5, 25, and 50 mg/ml of this extract. The minimal inhibitory concentration of green tea extract for S. mutans was 3.28 ± 0.7 mg/ml and for A. actinomycetemcomitans 6.25, for P. gingivalis and P. intermedia 12.5 mg/ml. CONCLUSIONS: Our findings showed that green tea extract exhibited strong antibacterial activity on S. mutans,A. actinomycetemcomitans,P. gingivalis and P. intermedia and therefore may be used in mouthwashes or dentifrices for prevention of dental caries and periodontal diseases.
Source: Medical Principles and Practice
DOI: 10.1159/000348299
Language: English
Topic: Tea Polyphenols and Effects
Subfield: Food Science
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W2009149233