Work

Extracellular Phosphate Alters Cementoblast Gene Expression

Year: 2006

Type: article

Abstract: Genetic data from humans and mice reveal that the formation of cementum is sensitive to intra- and extracellular phosphate/pyrophosphate distribution. The intracellular molecular pathways whereby altered levels of extracellular phosphate concentration may affect cementum formation have not been elucidated. To initiate inquiry, we have studied the temporal effects of extracellular phosphate on global patterns of gene expression in a line of immortalized mouse cementoblasts. Total RNA from cultured cementoblasts treated with 5 mM inorganic phosphate over a designated time period, from 1-48 hrs, was analyzed for global patterns of gene expression by means of DNA microarrays representing the complete mouse genome. Analyses of significant hybridization signals indicated that 5 mM extracellular phosphate alters the expression of genes comprising several gene ontology (GO) groups, including transcription factor activity and Wnt signaling.

Source: Journal of Dental Research

DOI: 10.1177/154405910608500605

Authors: R.B. Rutherford, B.L. Foster, T. Bammler, R.P. Beyer, S. Sato, M.J. Somerman

Institution: University of Washington

Language: English

Topic: Bone and Dental Protein Studies

Subfield: Rheumatology

Field: Medicine

Domain: Health Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of Environmental Health Sciences, National Institute of Dental and Craniofacial Research