Work

Genetically encoded unstrained olefins for live cell labeling with tetrazine dyes

Year: 2014

Type: article

Abstract: A number of non-canonical amino acids (NCAAs) with unstrained olefins are genetically encoded using mutant pyrrolysyl-tRNA synthetase-tRNA(Pyl)(CUA) pairs. These NCAAs readily undergo inverse electron-demand Diels-Alder cycloadditions with tetrazine dyes, leading to selective labeling of proteins bearing these NCAAs in live cells.

Source: Chemical Communications

DOI: 10.1039/c4cc06435f

Authors: Yan-Jiun Lee, Yadagiri Kurra, Yanyan Yang, Jessica Torres-Kolbus, Alexander Deiters, Wenshe R. Liu

Institutions: Texas A&M University, North Carolina State University, University of Pittsburgh

Language: English

Topic: Click Chemistry and Applications

Subfield: Organic Chemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funders: National Science Foundation, Welch Foundation, Institut National de la Santé et de la Recherche Médicale, National Cancer Institute