Work

Protein-Functionalized Polymer Brushes

Year: 2005

Type: article

Abstract: A new strategy for the preparation of protein-functionalized polymer brushes is reported, which is based on a combination of surface-initiated atom transfer radical polymerization (ATRP), p-nitrophenyl chloroformate activation of the surface hydroxyl groups, and subsequent O(6)-benzylguanine (BG) functionalization. The BG-functionalized brushes are used to chemoselectively immobilize O(6)-alkylguanine-DNA-alkyltransferase (AGT) fusion proteins with a defined orientation and surface density. These protein-modified polymer brushes are attractive candidates for the development of protein microarrays.

Source: Biomacromolecules

DOI: 10.1021/bm050016n

Authors: Stefano Tugulu, Anke Arnold, India Sielaff, Kai Johnsson, Harm-Anton Klok

Institution: École Polytechnique Fédérale de Lausanne

Language: English

Topic: Polymer Surface Interaction Studies

Subfield: Surfaces, Coatings and Films

Field: Materials Science

Domain: Physical Sciences

Open Access status: green

OpenAlex ID: W2009586206

FWCI: 11.3

Cites: 21

Cited by: 220

Related to: 10

Parts: 1