Work

Immunological identification and characterization of a delayed rectifier K+ channel polypeptide in rat brain.

Year: 1991

Type: article

Abstract: Antibodies specific for the drk1 polypeptide were used to characterize the corresponding protein in rat brain. Recombinant and synthetic immunogens containing fragments of the drk1 polypeptide were produced. Antibodies raised to these immunogens display monospecific reactions with the same 130-kDa polypeptide on immunoblots of adult rat brain membranes. Immunoprecipitation of 125I-labeled brain membranes identifies a 38-kDa peptide in tight association with the drk1 polypeptide. Immunohistochemical staining of sections of adult rat cortex shows that drk1 protein is restricted to neurons, where staining is present on dendrites and cell bodies but not on axons. These studies point to the value of such immunological reagents to the further characterization of the components of this delayed rectifier K+ channel in the mammalian central nervous system.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.88.23.10764

Author: J S Trimmer

Institution: Stony Brook University

Language: English

Topic: Ion channel regulation and function

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Heart, Lung, and Blood Institute, National Institute of Neurological Disorders and Stroke

Awards: MOLECULAR BIOPHYSICS AND BIOCHEMISTRY OF ION CHANNELS, VOLTAGE-REGULATED NA+ CHANNEL--STRUCTURE AND MECHANISMS