Work

Autoclaving as a chemical-free process to stabilize recombinant silk-elastinlike protein polymer nanofibers

Year: 2011

Type: article

Abstract: We report here that autoclaving is a chemical-free, physical crosslinking strategy capable of stabilizing electrospun recombinant silk-elastinlike protein (SELP) polymer nanofibers. Fourier transform infrared spectroscopy showed that the autoclaving of SELP nanofibers induced a conformational conversion of β-turns and unordered structures to ordered β-sheets. Tensile stress-strain analysis of the autoclaved SELP nanofibrous scaffolds in phosphate buffered saline at 37 °C revealed a Young's modulus of 1.02 ± 0.28 MPa, an ultimate tensile strength of 0.34 ± 0.04 MPa, and a strain at failure of 29% ± 3%.

Source: Applied Physics Letters

DOI: 10.1063/1.3604786

Authors: Weiguo Qiu, Joseph Cappello, Xiaoyi Wu

Institutions: University of Arizona, BIO5 Institute

Language: English

Topic: Silk-based biomaterials and applications

Subfield: Biomaterials

Field: Materials Science

Domain: Physical Sciences

Open Access status: bronze

Funder: National Institute of Biomedical Imaging and Bioengineering

Award: Microsystem-based Formation of Recombinant Protein Fibers