Work

13C6-[Benzene Ring]-Indole-3-Acetic Acid: A New Internal Standard for Quantitative Mass Spectral Analysis of Indole-3-Acetic Acid in Plants

Year: 1986

Type: article

Abstract: Indole-3-acetic acid (IAA) labeled with (13)C in the six carbons of the benzene ring is described for use as an internal standard for quantitative mass spectral analysis of IAA by gas chromatography/selected ion monitoring. [(13)C(6)]IAA was compared to the available deuterium labeled compounds and shown to offer the advantages of nonexchangeability of the isotope label, high isotopic enrichment, and chromatographic properties identical to that of the unlabeled compound. The utility of [(13)C(6)]IAA for measurement of endogenous IAA levels was demonstrated by analysis of IAA in Lemna gibba G-3.

Source: PLANT PHYSIOLOGY

DOI: 10.1104/pp.80.1.14

Authors: Jerry D. Cohen, Bruce G. Baldi, Janet Pernise Slovin

Institutions: Agricultural Research Service, United States Department of Agriculture, Washington State University, Beltsville Agricultural Research Center

Language: English

Topic: Mass Spectrometry Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: bronze

OpenAlex ID: W2010043131

FWCI: 7.752

Cites: 10

Cited by: 249

Related to: 10