Work

Suppression of Allelic Recombination and Aneuploidy by Cohesin Is Independent of Chk1 in Saccharomyces cerevisiae

Year: 2014

Type: article

Abstract: Sister chromatid cohesion (SCC), which is established during DNA replication, ensures genome stability. Establishment of SCC is inhibited in G2. However, this inhibition is relived and SCC is established as a response to DNA damage, a process known as Damage Induced Cohesion (DIC). In yeast, Chk1, which is a kinase that functions in DNA damage signal transduction, is considered an activator of SCC through DIC. Nonetheless, here we show that, unlike SCC mutations, loss of CHK1 did not increase spontaneous or damage-induced allelic recombination or aneuploidy. We suggest that Chk1 has a redundant role in the control of DIC or that DIC is redundant for maintaining genome stability.

Source: PLoS ONE

DOI: 10.1371/journal.pone.0113435

Authors: Shay Covo, Eric Chiou, Dmitry A. Gordenin, Michael A. Resnick

Institutions: National Institutes of Health, National Institute of Environmental Health Sciences

Language: English

Topic: DNA Repair Mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $2,382

Funders: U.S. Department of Health and Human Services, National Institutes of Health, National Institute of Environmental Health Sciences