Work

Production of recombinant SERA proteins of Plasmodium falciparum in Escherichia coli by using synthetic genes

Year: 1996

Type: article

Abstract: We expressed two regions of the serine repeat antigen (SERA) protein of Plasmodium falciparum in Escherichia coli by synthesizing the genes with a changed codon usage. One of the synthetic gene sequences encodes amino acid residues 17-382 (SE47') and the other encodes amino acid residues 586-802 (SE50A). The products produced by the synthetic gene sequences in E. coli accounted for 15-30% of the total bacterial protein. Antisera against both the purified gene products prepared in rats inhibited malaria parasite growth in vitro. The anti-SE47' serum was significantly more inhibitory than the anti-SE50A serum. The described methods provide a large scale preparation of recombinant antigens for improving and producing malaria vaccine.

Source: Vaccine

DOI: 10.1016/0264-410x(95)00238-v

Authors: Tomohiko Sugiyama, Kazutomo Suzue, Munehiro Okamoto, Joseph Inselburg, Kumiko Tai, Toshihiro Horii

Institutions: The University of Osaka, Research Institute for Microbial Diseases, The University of Osaka, Dartmouth College, Dartmouth Health

Language: English

Topic: Malaria Research and Control

Subfield: Public Health, Environmental and Occupational Health

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $3,620