Work

Thidiazuron-Induced High-Frequency Shoot Regeneration from Root Region of Robinia pseudoacacia L. Seedlings

Year: 2003

Type: article

Abstract: High-frequency regeneration of shoots was achieved at root region of seedlings of Robinia pseudoacacia L. cultured from seeds on medium supplemented with thidiazuron (TDZ, 1.0 μM). The roots of intact seedlings proliferated and formed a compact callus followed by differentiation of numerous shoots. Corresponding cultures on benzylaminopurine-containing medium exhibited much weaker response. Hypocotyl segments also formed shoots at a lower concentration of TDZ (0.1 μM). The shoots formed on TDZ-containing medium were well-developed and readily rooted on hormone-free medium. The obtained plants after acclimation in culture room survived after transfer to soil.

Source: Biologia Plantarum

DOI: 10.1023/b:biop.0000041068.19770.95

Authors: M. Hosseini-Nasr, A. Rashid

Institution: University of Delhi

Language: English

Topic: Plant tissue culture and regeneration

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Zero hunger

Open Access status: bronze

OpenAlex ID: W2012652884