Work

Facile functionalization of Ag@SiO2 core–shell metal enhanced fluorescence nanoparticles for cell labeling

Year: 2014

Type: article

Abstract: nanoparticles for live-cell imaging. The approach uses physical adsorption and does not need covalent linkage to synthesize antibody-based labels. The surface orientation is not controlled in this approach, but the signal enhancement is strong and consistent. Antibodies were then attached using a non-covalent process that takes advantage of biotin-avidin affinity. Metal-enhanced nanoparticles doped with rhodamine B were used as the luminescent reporter. The enhancement of rhodamine B was between 2.7-6.8 times. We demonstrated labeling of CD19+ Ramos B lymphocytes and CD4+ HuT 78 T lymphocytes using anti-CD19 and anti-CD4 nanocomposite labels, respectively. This physical adsorption process can accommodate a variety of fluorophore types, and has broad potential in bioanalytical and biosensing applications.

Source: Analytical Methods

DOI: 10.1039/c3ay42150c

Authors: Meicong Dong, Yu Tian, Dimitri Pappas

Institution: Texas Tech University

Language: English

Topic: Gold and Silver Nanoparticles Synthesis and Applications

Subfield: Electronic, Optical and Magnetic Materials

Field: Materials Science

Domain: Physical Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of General Medical Sciences, National Center for Research Resources

Awards: Rapid Single Molecule Detection System for Intracellular Protease Activity, Rapid Single Molecule Detection System for Intracellular Protease Activity