Year: 1969
Type: article
Abstract: A two‐dimensional fractionation procedure has been developed for separating radioactively‐labelled oligonucleotides of up to 50 residues long, using uniformly 32P‐labelled 5S RNA of Escherichia coli as a model compound. The method uses ionophoresis on cellulose acetate at pH 3.5 in the first dimension; and ascending chromatography with a concentrated mixture of oligonucleotides on thin layers of mixed DEAE‐cellulose and cellulose in the second dimension.
Source: European Journal of Biochemistry
Authors: G. G. Brownlee, F. Sanger
Institution: MRC Laboratory of Molecular Biology
Language: English
Subfield: Analytical Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: bronze
OpenAlex ID: W2012972361