Work

High-Resolution In Vivo Imaging of Fluorescent Proteins Using Window Chamber Models

Year: 2012

Type: book-chapter

Abstract: Fluorescent proteins enable in vivo characterization of a wide and growing array of morphological and functional biomarkers. To fully capitalize on the spatial and temporal information afforded by these reporter proteins, a method for imaging these proteins at high resolution longitudinally is required. This chapter describes the use of window chamber models as a means of imaging fluorescent proteins and other optical parameters. Such models essentially involve surgically implanting a window through which tumor or normal tissue can be imaged using existing microscopy techniques. This enables acquisition of high-quality images down to the cellular or subcellular scale, exploiting the diverse array of optical contrast mechanisms, while also maintaining the native microenvironment of the tissue of interest. This makes these techniques applicable to a wide array of problems in the biomedical sciences.

Source: Methods in molecular biology

DOI: 10.1007/978-1-61779-797-2_3

Authors: Gregory M. Palmer, Andrew N. Fontanella, Siqing Shan, Mark W. Dewhirst

Institutions: Duke University, Duke Medical Center

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Cancer Institute

Award: G4723046995