Work

High Efficiency Cross‐Reactive Monoclonal Antibody Production by Oral Immunization with Recombinant Norwalk Virus‐Like Particles

Year: 2006

Type: article

Abstract: Monoclonal antibodies (MAbs) are important for in-depth antigenic characterization and diagnosis of infections with human caliciviruses that cause almost all outbreaks of nonbacterial gastroenteritis. We compared different routes of immunization with nonreplicating virus-like particles (VLPs) from recombinant Norwalk virus (rNV) and recombinant Mexico virus (rMX) administered to BALB/c mice to determine the efficiency of hybridoma production. Oral immunization with VLPs without adjuvant resulted in high yields of MAb-secreting hybridomas (90%) to these VLPs of IgG (61%), IgM (29%) and IgA (10%) isotypes. Fusions with mesenteric lymph node lymphocytes yielded MAbs of various subclasses including IgG2a, IgG3, IgM and IgA. These results suggest that an immunization route that mimics the natural route of viral infection pathway may facilitate MAb technology by increasing the yields of antibody secreting hybridoma cells.

Source: Microbiology and Immunology

DOI: 10.1111/j.1348-0421.2006.tb03864.x

Authors: Tomoyuki Tanaka, Noritoshi Kitamoto, Xi Jiang, Mary K. Estes

Institutions: Sakai City Institute of Public Health, University of Hyogo, Cincinnati Children's Hospital Medical Center, Baylor College of Medicine

Language: English

Topic: Monoclonal and Polyclonal Antibodies Research

Subfield: Radiology, Nuclear Medicine and Imaging

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: bronze

Funders: National Institutes of Health, National Institute of Allergy and Infectious Diseases, National Institute of Diabetes and Digestive and Kidney Diseases