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G-Quadruplex-Modulated Fluorescence Detection of Potassium in the Presence of a 3500-Fold Excess of Sodium Ions

Year: 2010

Type: article

Abstract: A label-free detection of K(+) was developed using G-quadruplex DNA (c-Myc) modulated fluorescence enhancement of tetrakis-(diisopropylguanidino) zinc phthalocyanine (Zn-DIGP). Upon the addition of increasing concentrations of potassium, a detection limit of 0.8 μM for K(+) was easily achieved. Comparative titrations using sodium, lithium, ammonium, transition metal, or alkali earth salts revealed that the fluorescence enhancement was highly specific for potassium ions. This system has, for the first time, provided a means for detecting 40 μM of K(+) even in the presence of a 3500-fold excess of Na(+) ions.

Source: Analytical Chemistry

DOI: 10.1021/ac101894b

Authors: Haixia Qin, Jiangtao Ren, Jiahai Wang, Nathan W. Luedtke, Erkang Wang

Institutions: Chinese Academy of Sciences, University of Zurich, Changchun Institute of Applied Chemistry, State Key Laboratory of Electroanalytical Chemistry

Language: English

Topic: DNA and Nucleic Acid Chemistry

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W2013243779

FWCI: 3.905

Cites: 29

Cited by: 96

Related to: 10