Year: 2003
Type: article
Abstract: 13C NMR spectroscopy in combination with the infusion of (13)C-labeled precursors is currently the only technique that is capable of quantitatively studying energy metabolism, neurotransmission and other metabolic pathways non-invasively in vivo. (1)H-[(13)C]-NMR spectroscopy is a high-sensitivity alternative to direct (13)C NMR spectroscopy. The development of improved NMR methods for water suppression, spatial localization, broadband decoupling, shimming and signal quantification, together with the availability of high magnetic field strengths, has made (1)H-[(13)C]-NMR spectroscopy the method of choice for the detection of metabolism at a high spatial and/or temporal resolution. (1)H-[(13)C]-NMR spectroscopy can now be used to discriminate glutamatergic (excitatory) and GABAergic (inhibitory) neuronal activity. The improved sensitivity allows the detection of metabolism in different tissues (e.g. gray and white matter) and potentially even in smaller structures, like cortical layers. Finally, (1)H-[(13)C]-NMR spectroscopy allows the detection of energy metabolism and neurotransmission during functional activation, thereby further strengthening our understanding of the neurochemical basis of brain function.
Source: NMR in Biomedicine
DOI: 10.1002/nbm.847
Institution: Yale University
Language: English
Subfield: Radiology, Nuclear Medicine and Imaging
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
Funders: National Alliance for Research on Schizophrenia and Depression, National Institutes of Health, National Institute on Alcohol Abuse and Alcoholism, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute of Neurological Disorders and Stroke, National Institute of Biomedical Imaging and Bioengineering, Eunice Kennedy Shriver National Institute of Child Health and Human Development
Awards: G1304064820, G152221931, G2418547450, G2815518252, G2848036350, G3051910871, G3130311365, Hypoxia in Development; Injury and Adaptation Mechanisms, Quantitative MR Imaging and Spectroscopy in Alcoholism, G73399757, NMR Studies of GABA Metabolism and Regulation in Vivo, NMR Studies of Brain Energetics & Hypoglycemia In Vivo, G894358835, G9000145302
OpenAlex ID: W2013281941