Work

Transcription from a TATA-less promoter requires a multisubunit TFIID complex.

Year: 1991

Type: preprint

Abstract: In eukaryotes, the TATA box-binding protein (TBP) is responsible for nucleating assembly of the transcription initiation machinery. Here, we report that a TFIID complex containing TBP is essential for transcription even at a promoter that lacks a TATA box. Immunopurification of TFIID reveals that the active species in reconstituting TATA-less transcription is a multisubunit complex consisting of TBP and many TBP-associated factors (TAFs).

Source: Genes & Development

DOI: 10.1101/gad.5.11.1935

Authors: B F Pugh, R Tjian

Institutions: Howard Hughes Medical Institute, University of California, Berkeley, Pennsylvania State University

Language: English

Topic: Genomics and Chromatin Dynamics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funder: National Institutes of Health

OpenAlex ID: W2013980278

Cites: 40

Cited by: 590

Related to: 10