Nonintegrating Knockdown and Customized Scaffold Design Enhances Human Adipose-Derived Stem Cells in Skeletal Repair
Year: 2011
Type: article
Abstract: An urgent need exists in clinical medicine for suitable alternatives to available techniques for bone tissue repair. Human adipose-derived stem cells (hASCs) represent a readily available, autogenous cell source with well-documented in vivo osteogenic potential. In this article, we manipulated Noggin expression levels in hASCs using lentiviral and nonintegrating minicircle short hairpin ribonucleic acid (shRNA) methodologies in vitro and in vivo to enhance hASC osteogenesis. Human ASCs with Noggin knockdown showed significantly increased bone morphogenetic protein (BMP) signaling and osteogenic differentiation both in vitro and in vivo, and when placed onto a BMP-releasing scaffold embedded with lentiviral Noggin shRNA particles, hASCs more rapidly healed mouse calvarial defects. This study therefore suggests that genetic targeting of hASCs combined with custom scaffold design can optimize hASCs for skeletal regenerative medicine.
Source: Stem Cells
DOI: 10.1002/stem.757
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: bronze
Awards: Nanostructuring and Molecular Imaging of Engineered Cardiovascular Tissues, G1579562262, Calvarial Regeneration using Biomatrix-Encapsulated Skeletal Progenitors, G2238114474, G3911217622, Enhanced Calvarial Regeneration Via RNAi-Mediated Suppression of BMP Antagonism, G4808615701, G8668468096, G9000117819
OpenAlex ID: W2014051301