Enhanced survival of rat neonatal cerebral cortical neurons at subatmospheric oxygen tensions in vitro
Year: 1986
Type: article
Abstract: In vitro tissue culture experiments are routinely performed at atmospheric oxygen tension (21%), although most cells experience lower oxygen tensions in vivo. The optimal oxygen tension for the survival of neurons and synthesis of neurofilament (NF) was determined using dissociated neonatal rat cerebral cortex and monoclonal antibodies to the 68 kDa and 200 kDa subunits of NF. Maximum production of each subunit determined by Enzyme-Linked Immunosorbant Assay occurred at 9% O2. Neuronal survival determined by immunohistochemistry was also maximal at 9% O2. CNS neurons are highly sensitive to oxygen tension and grow best at levels approximating capillary rather than atmospheric pO2.
Source: Brain Research
Authors: Frederick S. Kaplan, Carl T. Brighton, Mark J. Boytim, Michael E. Selzer, Virginia Lee, Kurt Spindler, Donald Silberberg, Jonathan Black
Institution: University of Pennsylvania
Language: English
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
Estimated APC paid: $3,360
OpenAlex ID: W2014488454