Year: 2009
Type: article
Abstract: The cell-material interaction is an important factor to be considered in the applications of biomaterials. The properties of biomaterials, for example crystallinity, would change the cell behaviors such as proliferation, differentiation, morphology and so on. In this article, the research was focused on the relationship between the crystallinity of polymeric substrates and the cell responses. Two kinds of biodegradable poly(L-lactide) (PLLA) films with different crystallinity were prepared and used as substrates for cell behavior study. The PLLA films were fabricated with crystallinity of 19% and 69% through solution casting and isothermal processing, respectively. The crystallinity of PLLA films was characterized by DSC and XRD. The surface morphologies of PLLA films with different crystallinity were observed by SEM. Bone marrow stromal cells (MSCs) isolated from the marrow of new born male Sprague Dawley rats were cultured on the films. The cell morphology, proliferation, total protein amount and alkaline phosphatase (ALP) activities were tested. The results showed that the crystallinity of PLLA films affected the behavior of MSCs greatly.
Source: e-Polymers
Authors: Qiangying Yi, Xiantao Wen, Li Li, Bin He, Yao Wu, Yu Nie, Zhongwei Gu
Institution: Sichuan University
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: bronze
OpenAlex ID: W2015039261