Work

CTG Triplet Repeats from Human Hereditary Diseases Are Dominant Genetic Expansion Products in Escherichia coli

Year: 1996

Type: article

Abstract: The relative ability of the 10 triplet repeat sequences to be expanded in Escherichia coli was determined. Surprisingly, CTG tracts are expanded at least 8 times more frequently than any of the other nine triplets. Low levels of expansion were found also for CGG, GTG, and GTC. Thus, the structure of the CTG repeats and/or their utilization by the DNA synthetic systems in vivo must be quite different from the other triplets. These data further validate this genetically defined system for elucidating molecular mechanisms of expansion and may explain why most triplet repeat hereditary neuromuscular and neurodegenerative disease genes contain CTG repeats.

Source: Journal of Biological Chemistry

DOI: 10.1074/jbc.271.4.1853

Authors: Keiichi Ohshima, Seongman Kang, Robert D. Wells

Institutions: Texas Medical Center, Texas A&M University

Language: English

Topic: Genetic Neurodegenerative Diseases

Subfield: Cellular and Molecular Neuroscience

Field: Neuroscience

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $3,430

Funders: National Science Foundation, Welch Foundation, National Institutes of Health, National Institute of General Medical Sciences