Work

Circulating human CD4 and CD8 T cells do not have large intracellular pools of CCR5

Year: 2010

Type: article

Abstract: CC Chemokine Receptor 5 (CCR5) is an important mediator of chemotaxis and the primary coreceptor for HIV-1. A recent report by other researchers suggested that primary T cells harbor pools of intracellular CCR5. With the use of a series of complementary techniques to measure CCR5 expression (antibody labeling, Western blot, quantitative reverse transcription polymerase chain reaction), we established that intracellular pools of CCR5 do not exist and that the results obtained by the other researchers were false-positives that arose because of the generation of irrelevant binding sites for anti-CCR5 antibodies during fixation and permeabilization of cells.

Source: Blood

DOI: 10.1182/blood-2010-05-282509

Authors: Heather A. Pilch-Cooper, Scott F. Sieg, Thomas J. Hope, Ann Koons, Jean-Michel Escola, Robin Offord, Ronald S. Veazey, Donald E. Mosier, Brian Clagett, Kathy Medvik, Julie K. Jadlowsky, Mark R. Chance, Janna G. Kiselar, James A. Hoxie, Ronald G. Collman, Nadeene E. Riddick, Valentina Mercanti, Oliver Hartley, Michael M. Lederman

Institutions: University Hospitals Cleveland Medical Center, Case Western Reserve University, Northwestern University, University of Geneva, Mintaka Foundation for Medical Research, Tulane University, Scripps Research Institute, University of Pennsylvania

Language: English

Topic: Chemokine receptors and signaling

Subfield: Oncology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

Funders: James B. Pendleton Charitable Trust, Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung, National Institutes of Health, National Cancer Institute, National Institute of Allergy and Infectious Diseases

Awards: G5418471891, Basic and Comparative Studies of CCR5 Inhibition to Prevent HIV Transmission, G8690818240, Case Comprehensive Cancer Center Support Grant, Rustbelt Center for AIDS Research (Case/UHC-Pitt CFAR), Tulane National Primate Research Center, Penn Center for AIDS Research

OpenAlex ID: W2015385795

FWCI: 0.746

Cites: 21

Cited by: 9

Related to: 10