Work

In Vitro Synthesis of Procollagen on Polysomes

Year: 1972

Type: article

Abstract: The major collagenous products synthesized in a cell-free polysome preparation are pro-alpha1 and pro-alpha2, formed in a ratio of 2:1. They are the precursor forms of alpha1 and alpha2 chains of normal connective-tissue collagen that are also formed in small amounts. A procollagen peptidase activity has been demonstrated in the supernatant fraction that can account for the formation of alpha1 and alpha2 chains from their precursors. The polysomal system is activated by a salt extract of reticulocyte ribosomes and is inhibited by aurintricarboxylic acid, suggesting that the polysomes are able to initiate protein synthesis.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.69.9.2727

Authors: S. S. Kerwar, Leonard D. Kohn, Charles M. Lapiere, Herbert Weissbach

Institutions: National Institutes of Health, University of Liège

Language: English

Topic: RNA and protein synthesis mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W2015427760

FWCI: 3.717

Cites: 17

Cited by: 33

Related to: 10