Induction of intestinal sucrase-isomaltase in suckling rats after hydrocortisone injections and substrate administration
Year: 1979
Type: article
Abstract: Upjohn Laboratories) at days 10 (1 mg/rat), 11 and 12 (0.5 mg/rat), and the remaining animals serving as controls.Substrate administration.-Two groups (1 and 2) of 4 suckling rats each were removed from the mother at day 11 and were bottle-fed every 4 hrs until day 14.The basic diet (Raul et al., 1978b) was supplemented with 13 p. 100 sucrose (Group 1) or 13 p. 100 lactose (Group 2).Group 3 included the rest of the litter and had normal access to maternal milk.Separation and characterization of brush border enzymes.-Partial purification of intestinal brush border membranes and separation of brush border protein and enzyme assays were performed as described elsewhere (Raul et al., 1978a).Results.In all experimental control groups, the protein patterns were similar and no sucrase-isomaltase was detected on the gels.The major protein band located in position 5 corresponded to lactase activity (fig.1a).
DOI: 10.1051/rnd:19790616
Authors: F. RAUL, Patricia SIMON, Michèle KEDINGER, Katy HAFFEN
Institution: Inserm
Language: English
Subfield: Genetics
Domain: Life Sciences
SDG: Zero hunger
Open Access status: bronze
OpenAlex ID: W2015572946