Year: 1994
Type: article
Abstract: Okadaic acid (OA) induced marked shape changes of human platelets but neither granule secretion nor increase in [Ca2+]i occurred. Morphological changes induced by OA were unique and different from those observed with thrombin mainly in two respects. One is that the pseudopods formed by OA treatment were longer and straighter than those by thrombin. The other is that no granule centralization was observed with OA-treatment, although central condensation of cytofilaments was observed as was with thrombin. The immunocytochemical analysis employing anti-myosin antibody revealed that myosin was distributed in the elongated pseudopods. Since OA induced phosphorylation of the 20-kDa myosin light chain (MLC20) corresponding to the change in shape of platelets but did not induce phosphorylation of the 47-kDa protein (pleckstrin) (Higashihara M. et al. FEBS Lett. 307:206-210, 1992), these results suggest that phosphorylation of MLC20 plays an important role in OA-induced unique morphological changes of platelets.
DOI: 10.1540/jsmr.30.57
Authors: Hayato KAWAKAMI, Masaaki HIGASHIHARA, Xiao-Hui SONG, Kiyoshi KUROKAWA, Mitsuo IKEBE, Hiroshi HIRANO
Language: English
Subfield: Hematology
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W2015813659