Work

Characterization and identification of compost bacteria based on 16S rRNA gene sequencing

Year: 2012

Type: article

Abstract: The aim of this study was to isolate and characterize bacteria from the compost of fruit and vegetable waste (FVW) for plant growth-promoting (PGP) activities and investigate the pro-active influence of bacterial isolates on wheat growth. Fourteen bacterial strains (RHC-1 to RHC-14) were isolated and purified in tryptic soya agar (TSA). In addition to being biochemically characterized, these bacterial strains were also tested for their PGP traits, such as phosphate (P)-solubilization, nif H gene amplification, indole-3-acetic acid (IAA) quantification and the production of ammonia, oxidase and catalase. Based on 16S rRNA gene sequencing, these bacterial strains were identified as belonging to species of Bacillus , Lysinibacillus , Lysobacter , Staphylococcus , Enterobacter , Pseudomonas and Serratia . All bacterial strains solubilized tri-calcium phosphate and produced IAA. Two bacterial strains RHC-8 ( Enterobacter sp.) and RHC-13 ( Pseudomonas sp.) solubilized the maximum amount of tri-calcium phosphate, i.e. 486 and 464 μg/ml, respectively. P-solubilization was associated with a significant drop in the pH of the broth culture from an initial pH of 7 to pH 4.43. In addition to P-solubilization and IAA production, six bacterial strains also carried the nif H gene and were further evaluated for their effect on wheat ( Triticum aestivum ) growth under controlled conditions. All six bacterial strains enhanced wheat growth as compared to uninoculated control plants. Two of the bacterial strains, RHC-8 and RHC-13, identified as Enterobacter aerogenes and Pseudomonas brenneri , respectively, were assessed as potential PGP rhizobacteria due to exhibiting characteristics of four or more PGP traits and enhancing wheat growth though their specific mechanism of action.

Source: Annals of Microbiology

DOI: 10.1007/s13213-012-0542-4

Authors: Rifat Hayat, Rizwan Ali Sheirdil, Muhammad Iftikhar-ul-Hassan, Iftikhar Ahmed

Institution: Pir Mehr Ali Shah Arid Agriculture University

Language: English

Topic: Plant-Microbe Interactions and Immunity

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Zero hunger

Open Access status: gold

Estimated APC paid: $2,290