Work

Trypsin-Like Nature of the Pancreatic Factor That Corrects Vitamin B12 Malabsorption Associated with Pancreatic Dysfunction

Year: 1973

Type: article

Abstract: Hog pancreas was subfractionated and assessed for its ability to correct vitamin B(12) malabsorption in patients with pancreatic dysfunction and in rats with partial pancreatic extirpation. The constituent obtained from the pancreas that increased vitamin B(12) absorption in both humans and rats was soluble at 50,000 g, heat labile, acid stable, and approximately 20,000-25,0000 in molecular weight. The active subfractions contained tryptic and chymotryptic but no amylase or lipase activity. Thrice-crystallized trypsin corrected the vitamin B(12) malabsorption in both patients with pancreatic insufficiency and in rats with subtotal pancreatectomy. These data indicate that pancreatic proteolytic enzymes-in particular, trypsin-are necessary for optimal vitamin B(12) absorption.

Source: Journal of Clinical Investigation

DOI: 10.1172/jci107346

Authors: Phillip P. Toskes, Julius J. Deren, Marcel E. Conrad

Institutions: Walter Reed National Military Medical Center, University of Pennsylvania, Walter Reed Army Institute of Research

Language: English

Topic: Folate and B Vitamins Research

Subfield: Nutrition and Dietetics

Field: Nursing

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: bronze

OpenAlex ID: W2016839052

FWCI: 5.276

Cites: 15

Cited by: 44

Related to: 10