Work

Peptidyl-Puromycin Synthesis on Polyribosomes from Escherichia coli

Year: 1972

Type: article

Abstract: Peptide bond synthesis was studied with native polyribosomes of E. coli. With the use of this system for transpeptidation, it was possible to show that a single K(+) activates the ribosome monomers of polyribosomes; that protonation of a single group (probably imidazole or an N-terminal amino group) with a pK(a) equal to about 7.2 inactivates the transpeptidase complex; that Mn(++) can substitute for Mg(++), but that Ca(++), spermidine, and putrescine do so only very poorly; and that the K(m) for puromycin in this system is about 2.4 x 10(-6) M.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.69.3.624

Author: Sidney Pestka

Language: English

Topic: RNA and protein synthesis mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W2016857063

FWCI: 3.328

Cites: 31

Cited by: 65

Related to: 10