Work

A Pulsed-Field Gel Electrophoresis (PFGE) Map of Twelve Loci on Chromosome 11q11-q13

Year: 1993

Type: article

Abstract: We report a pulsed-field gel electrophoresis map of 12 loci on proximal human chromosome 11q. Linkage studies have shown that this region of chromosome 11 contains the genes for familial atopic disease (APY) and multiple endocrine neoplasia type I (MEN1) (4). A physical map containing polymorphic loci will aid in the isolation of these disease genes. The map reported here has two noncontiguous groups of loci accounting for 8 of the 12 loci evaluated. One group spans a maximum distance of 1600 kb and includes D11S146, BCL1, PRAD1, INT2, and HSTF1. The other group includes FTH1, C1NH, and COX8. TCN1, PGA, and PYGM did not yield any comigrating fragments and could not be physically linked on this PFGE map. These data enhance previously published physical maps of proximal 11q by refining the localization of and distances between markers in the BCL1 region. Additionally, new information about the locations and physical relationships between FTH1, C1NH, and COX8 is presented.

Source: Genomics

DOI: 10.1006/geno.1993.1080

Authors: E.M. Petty, A. Arnold, S.J. Marx, A.E. Bale

Institutions: National Institutes of Health, Harvard University, Yale University, Massachusetts General Hospital, National Institute of Diabetes and Digestive and Kidney Diseases

Language: Chinese

Topic: Genetic Associations and Epidemiology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $2,980