Work

Enzymatic cleavage of uracil-containing single-stranded DNA linkers for the efficient release of affinity-selected circulating tumor cells

Year: 2015

Type: article

Abstract: We report a novel strategy to enzymatically release affinity-selected cells, such as circulating tumor cells (CTCs), from surfaces with high efficiency (∼90%) while maintaining cell viability (>85%). The strategy utilizes single-stranded DNAs that link a capture antibody to the surfaces of a CTC selection device. The DNA linkers contain a uracil residue that can be cleaved.

Source: Chemical Communications

DOI: 10.1039/c4cc09765c

Authors: Soumya V. Nair, Małgorzata A. Witek, Joshua M. Jackson, Maria A. M. Lindell, Sally A. Hunsucker, Travis Sapp, Caroline E. Perry, Mateusz L. Hupert, Victoria Bae-Jump, Paola A. Gehrig, Weiya Z. Wysham, Paul M. Armistead, Peter Voorhees, Steven A. Soper

Institutions: University of North Carolina at Chapel Hill, UNC Lineberger Comprehensive Cancer Center, Louisiana State University, Ulsan National Institute of Science and Technology

Language: English

Topic: Advanced biosensing and bioanalysis techniques

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Institutes of Health, National Cancer Institute, National Institute of Biomedical Imaging and Bioengineering

Awards: G1411983911, Acute Myeloid Leukemia: MRD Analysis Using Modular uFluidics and uFlow Cytometry, Biotechnology Resource Center of BioModular Multi-scale Systems (CBM2) for Precision Medicine

OpenAlex ID: W2017055418

FWCI: 2.058

Cites: 28

Cited by: 33

Related to: 10