Work

Perturbation of the conformational equilibria in Ras by selective mutations as studied by 31P NMR spectroscopy

Year: 2004

Type: article

Abstract: Ras regulates a variety of different signal transduction pathways acting as molecular switch. It was shown by liquid and solid-state (31)P NMR spectroscopy that Ras exists in the guanosine-5'-(beta,gamma-imido)triphosphate bound form in at least two conformational states interconverting in millisecond time scale. The relative population between the two conformational states affects drastically the affinity of Ras to its effectors. (31)P NMR spectroscopy shows that the conformational equilibrium can be shifted specifically by point mutations, including mutations with oncogenic potential, thus modifying the effector interactions and their coupling to dynamic properties of the protein.

Source: FEBS Letters

DOI: 10.1016/j.febslet.2004.11.020

Authors: Michael Spoerner, Alfred Wittinghofer, Hans Robert Kalbitzer

Institutions: University of Regensburg, Max Planck Institute of Molecular Physiology

Language: English

Topic: Protein Kinase Regulation and GTPase Signaling

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funder: Deutsche Forschungsgemeinschaft

Award: G1005310647