Work

Liquid chromatographic separation of enantiomers of β-amino acids using a chiral stationary phase

Year: 1986

Type: article

Abstract: The enantiomers of both alpha-substituted beta-alanines and beta-substituted beta-alanines may be chromatographically separated using silica-bonded chiral stationary phases derived from N-acetylated alpha-arylalkylamines. The amino acids are chromatographed as alkyl esters of N-3,5-dinitrobenzoyl derivatives; separability factors range from 1.11 to 1.65 for nine alpha-substituted beta-alanines and from 1.08 to 1.20 for nine beta-substituted beta-alanines. The enantiomers of beta-aminoisobutyrate and beta-leucine, chiral beta-amino acids occurring in animal tissues and physiological fluids, are among those resolved. The enantiomers of R,S-beta-aminoisobutyrate and several related alpha-alkyl-beta-alanines were prepared by chromatographic resolution of diastereomeric dipeptides.

Source: Journal of Chromatography A

DOI: 10.1016/s0021-9673(01)86986-7

Authors: Owen W. Griffith, Ernest B. Campbell, William H. Pirkle, Athanasios Tsipouras, Myung Ho Hyun

Institutions: Cornell University, University of Illinois Urbana-Champaign, University of Illinois System

Language: English

Topic: Analytical Chemistry and Chromatography

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: hybrid

Estimated APC paid: $4,040

Funders: National Science Foundation, Foundation for the National Institutes of Health