Work

Cloning of cDNA sequences of hormone-regulated genes from the MCF-7 human breast cancer cell line

Year: 1982

Type: article

Abstract: cDNA clones corresponding to a mRNA whose level is rapidly increased by addition of oestradiol to the culture medium have been isolated by differential screening of a cDNA library from the breast cancer cell line MCF-7, which contains oestrogen receptors. Such clones will be useful in studies of the DNA sequences required for hormonal induction and to determine whether expression of the corresponding gene is in any way related to the cancerous state. We have also obtained a cDNA clone for a messenger whose level is apparently decreased by steroid hormones.

Source: Nucleic Acids Research

DOI: 10.1093/nar/10.24.7895

Authors: P. Masiakowski, R. Breathnach, J. Bloch, F. Gannon, A. Krust, P. Chambon

Institutions: Centre National de la Recherche Scientifique, Inserm

Language: English

Topic: Estrogen and related hormone effects

Subfield: Endocrinology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

Funders: Institut National de la Santé et de la Recherche Médicale, Centre National de la Recherche Scientifique