Tissue spheroid fusion-based in vitro screening assays for analysis of tissue maturation
Year: 2010
Type: article
Abstract: Organ printing or computer-aided robotic layer-by-layer additive biofabrication of thick three-dimensional (3D) living tissue constructs employing self-assembling tissue spheroids is a rapidly evolving alternative to classic solid scaffold-based approaches in tissue engineering. However, the absence of effective methods of accelerated tissue maturation immediately after bioprinting is the main technological imperative and potential impediment for further progress in the development of this emerging organ printing technology. Identification of the optimal combination of factors and conditions that accelerate tissue maturation ('maturogenic' factors) is an essential and necessary endeavour. Screening of maturogenic factors would be most efficiently accomplished using high-throughput quantitative in vitro tissue maturation assays. We have recently reviewed the formation of solid scaffold-free tissue constructs through the fusion of bioprinted tissue spheroids that have measurable material properties. We hypothesize that the fusion kinetics of these tissue spheroids will provide an efficacious in vitro assay of the level of tissue maturation. We report here the results of experimental testing of two simple quantitative tissue spheroid fusion-based in vitro high-throughput screening assays of tissue maturation: (a) a tissue spheroid envelopment assay; and (b) a tissue spheroid fusion kinetics assay.
DOI: 10.1002/term.291
Authors: Zoltan Hajdu, Vladimir Mironov, Agnes Nagy Mehesz, Russell A. Norris, Roger R. Markwald, Richard P. Visconti
Institution: Medical University of South Carolina
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: green
Awards: G1761438407, South Carolina IDeA Network of Biomedical Research Excellence, EXTRAMURAL RESEARCH FACILITIEIS CONSTRUCTION, SC COBRE FOR DEVELOPMENTALLY BASED CARDIOVASCULAR DISEASES, G2584952555, G2598401476, G3281524897, G350062807, General Clinical Research Center, G529529811, EXTRAMURAL RESEARCH FACILITIES CONSTRUCTION
OpenAlex ID: W2018712244