Work

Staphylococcus aureus Peptidoglycan Tertiary Structure from Carbon-13 Spin Diffusion

Year: 2009

Type: article

Abstract: The cell-wall peptidoglycan of Staphylococcus aureus is a heterogeneous, highly cross-linked polymer of unknown tertiary structure. We have partially characterized this structure by measuring spin diffusion from (13)C labels in pentaglycyl cross-linking segments to natural-abundance (13)C in the surrounding intact cell walls. The measurements were performed using a version of centerband-only detection of exchange (CODEX). The cell walls were isolated from S. aureus grown in media containing [1-(13)C]glycine. The CODEX spin diffusion rates established that the pentaglycyl bridge of one peptidoglycan repeat unit of S. aureus is within 5 A of the glycan chain of another repeat unit. This surprising proximity is interpreted in terms of a model for the peptidoglycan lattice in which all peptide stems in a plane perpendicular to the glycan mainchain are parallel to one another.

Source: Journal of the American Chemical Society

DOI: 10.1021/ja808971c

Authors: Shasad Sharif, Manmilan Singh, Sung Joon Kim, Jacob Schaefer

Institution: Washington University in St. Louis

Language: English

Topic: Advanced NMR Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funder: National Institute of Biomedical Imaging and Bioengineering

Award: GLYCOPEPTIDE COMPLEXES WITH BACTERIAL CELL WALLS BY REDOR NMR